pMC_0_4_35_cpf1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 633
Illegal XbaI site found at 1356
Illegal SpeI site found at 3622 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1713
Illegal NheI site found at 3433
Illegal SpeI site found at 3622 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 3494
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 633
Illegal XbaI site found at 1356
Illegal SpeI site found at 3622 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 633
Illegal XbaI site found at 1356
Illegal SpeI site found at 3622 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part was aquired via PCR with primers that have BsmbI cutting sites as overhang. The overhangs were chosen to anneal to the backbone of the digested Lvl0 entry vector.
Source
The part was aquired via PCR from the vector pSL2680, which derived from RSF1010.